Torres, Y.R., Bugni, T.S., Berlinck, R.G.S., Ireland, C.M., Magalhães, A., Ferreira, A.G., Rocha, R.M., “Sebastianines A and B, Novel Biologically Active Pyridoacridine Alkaloids from the Brazilian Ascidian Cystodytes dellechiajei”, J. Org. Chem., 67, 5429-5432 (2002).

Abstract : Fractionation of the crude methanol extract of the ascidian Cystodytes dellechiajei collected in Brazil yielded two novel alkaloids, sebastianine A (1) and sebastianine B (2). The structures of both 1 and 2 were established by analysis of spectroscopic data, indicating an unprecedented ring system for both compounds, comprising a pyridoacridine system fused with a pyrrole unit in sebastianine A (1) and a pyridoacridine system fused with a pyrrolidine system condensed with a-hydroxyisovaleric acid in sebastianine B (2). Both alkaloids displayed a cytotoxic profile against a panel of HCT-116 colon carcinoma cells indicative of a p53 dependant mechanism.